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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
18/12/2020 |
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Actualizado : |
18/12/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
DORSCH, M.; REGIDOR-CERRILLO, J.; ORTEGA-MORA, L.M.; SCIOLI, M.V.; MORRELL, E.L.; CANTÓN, G.J.; MOORE, D.P.; HECKER, Y.P. |
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Afiliación : |
MATÍAS ANDRÉS DORSCH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay;Faculty of Agrarian Sciences, National University of Mar del Plata (UNMdP), Mar del Plata, Argentina; JAVIER REGIDOR-CERRILLO, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Madrid, Spain.; LUIS M. ORTEGA-MORA, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Madrid, Spain.; MARÍA V. SCIOLI, María V. Scioli National Institute of Agrarian Technology (INTA), Buenos Aires, Argentina.; ELEONORA L. MORRELL, National Institute of Agrarian Technology (INTA), Buenos Aires, Argentina.; GERMÁN J. CANTÓN, National Institute of Agrarian Technology (INTA), Buenos Aires, Argentina.; DADÍN P. MOORE, Faculty of Agrarian Sciences, National University of Mar del Plata (UNMdP), Mar del Plata, Argentina.; YANINA P. HECKER, National Institute of Agrarian Technology (INTA), Buenos Aires, Argentina. |
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Título : |
Microsatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Parasitology Research, December 2020, Volume 119, Issue 12, Pages 4049-4059. Doi: https://doi-org/10.1007/s00436-020-06922-x |
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DOI : |
10.1007/s00436-020-06922-x |
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Idioma : |
Inglés |
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Notas : |
Article history:Received: 12 August 2020 /Accepted: 5 October 2020 |
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Contenido : |
Abstract: Neospora caninum is an apicomplexan protozoan and a major cause of abortion in cattle worldwide. In the Argentinian Humid Pampa, bovine neosporosis causes severe economic losses. Despite this, information on the genetic structure of N. caninum in this region is limited. Therefore, this study aimed to genetically characterize N. caninum isolates associated with bovine abortion in the Humid Pampa region. For this purpose, spontaneous bovine fetal tissues submitted for diagnosis to the Veterinary Diagnostic Service at INTA Balcarce during 2008?2019 were assessed by PCR, indirect fluorescent antibody test (IFAT), and histologic analysis. PCR-positive samples were tested by multilocus microsatellite genotyping (MLGs) using 9 microsatellite markers. Thirty-one different genotypes were identified from 32 samples with at least seven markers. Argentinian MLGs were grouped into two clonal clusters when analyzed using eBURST network and principal coordinate analysis. No segregation based on the year of collection, animal biotype, or geographic origin was observed. In addition, the presence of linkage disequilibrium supported the clonal propagation of Argentinian MLGs. One Argentinian subpopulation was associated with isolates from Spain, Uruguay, Brazil, and Mexico, and the other one was linked to isolates from Scotland, Spain, and Germany. These findings reveal the presence of two clonal subpopulations of N. caninum in the Humid Pampa. |
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Palabras claves : |
ABORTION; BOVINE; MICROSATELLITE MARKERS; NEOSPORA CANINUM; PLATAFORMA SALUD ANIMAL. |
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Thesagro : |
ARGENTINA. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02492naa a2200301 a 4500
001 1061592
005 2020-12-18
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1007/s00436-020-06922-x$2DOI
100 1 $aDORSCH, M.
245 $aMicrosatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina.$h[electronic resource]
260 $c2020
500 $aArticle history:Received: 12 August 2020 /Accepted: 5 October 2020
520 $aAbstract: Neospora caninum is an apicomplexan protozoan and a major cause of abortion in cattle worldwide. In the Argentinian Humid Pampa, bovine neosporosis causes severe economic losses. Despite this, information on the genetic structure of N. caninum in this region is limited. Therefore, this study aimed to genetically characterize N. caninum isolates associated with bovine abortion in the Humid Pampa region. For this purpose, spontaneous bovine fetal tissues submitted for diagnosis to the Veterinary Diagnostic Service at INTA Balcarce during 2008?2019 were assessed by PCR, indirect fluorescent antibody test (IFAT), and histologic analysis. PCR-positive samples were tested by multilocus microsatellite genotyping (MLGs) using 9 microsatellite markers. Thirty-one different genotypes were identified from 32 samples with at least seven markers. Argentinian MLGs were grouped into two clonal clusters when analyzed using eBURST network and principal coordinate analysis. No segregation based on the year of collection, animal biotype, or geographic origin was observed. In addition, the presence of linkage disequilibrium supported the clonal propagation of Argentinian MLGs. One Argentinian subpopulation was associated with isolates from Spain, Uruguay, Brazil, and Mexico, and the other one was linked to isolates from Scotland, Spain, and Germany. These findings reveal the presence of two clonal subpopulations of N. caninum in the Humid Pampa.
650 $aARGENTINA
653 $aABORTION
653 $aBOVINE
653 $aMICROSATELLITE MARKERS
653 $aNEOSPORA CANINUM
653 $aPLATAFORMA SALUD ANIMAL
700 1 $aREGIDOR-CERRILLO, J.
700 1 $aORTEGA-MORA, L.M.
700 1 $aSCIOLI, M.V.
700 1 $aMORRELL, E.L.
700 1 $aCANTÓN, G.J.
700 1 $aMOORE, D.P.
700 1 $aHECKER, Y.P.
773 $tParasitology Research, December 2020, Volume 119, Issue 12, Pages 4049-4059. Doi: https://doi-org/10.1007/s00436-020-06922-x
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INIA La Estanzuela (LE) |
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
20/11/2015 |
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Actualizado : |
09/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
CAJARVILLE, C.; BRITOS, A.; ERRANDONEA, N.; GUTIÉRREZ, L.; COZZOLINO, D.; REPETTO, J.L. |
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Afiliación : |
DANIEL COZZOLINO GÓMEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Diurnal changes in water-soluble carbohydrate concentration in lucerne and tall fescue in autumn and the effects on in vitro fermentation. |
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Complemento del título : |
Research Article. |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
New Zealand Journal of Agricultural Research, 2015, v. 58, no.3, p. 281-291. |
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ISSN : |
0028-8233 (Print) / 1175-8775 (Online) |
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DOI : |
10.1080/00288233.2015.1018391 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 23 January 2014 // Accepted 5 February 2015. |
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Contenido : |
ABSTRACT.
The aim of the experiment was to study diurnal variation in water-soluble carbohydrate (WSC) concentrations in leaf and stem of tall fescue and lucerne in autumn and to determine the impact of these changes on the rate and the extent of in vitro fermentation. Vegetative herbage of lucerne and tall fescue were sampled at 0900, 1300 and 1700 h, following three consecutive regrowths between April and May. Whole plants, leaf and stem were analysed for chemical composition and in vitro gas production. There was a linear increase in WSC throughout the day for all plants and plant components (P < 0.001). In both fescue and lucerne, this rise resulted in an increased rate of gas production, although this relationship was more pronounced in fescue. Use of time of day to govern grazing management decisions to potentially improve the rumen supply of WSC and nitrogen in autumn can be achieved from tall fescue or lucerne pastures.
© 2015 The Royal Society of New Zealand |
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Palabras claves : |
HARVESTING TIME; RUMINAL FERMENTATION; SUGARS. |
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Thesagro : |
FESTUCA ARUNDINACEA; MEDICAGO SATIVA. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 01947naa a2200277 a 4500
001 1053972
005 2019-10-09
008 2015 bl uuuu u00u1 u #d
022 $a0028-8233 (Print) / 1175-8775 (Online)
024 7 $a10.1080/00288233.2015.1018391$2DOI
100 1 $aCAJARVILLE, C.
245 $aDiurnal changes in water-soluble carbohydrate concentration in lucerne and tall fescue in autumn and the effects on in vitro fermentation.$h[electronic resource]
260 $c2015
500 $aArticle history: Received 23 January 2014 // Accepted 5 February 2015.
520 $aABSTRACT. The aim of the experiment was to study diurnal variation in water-soluble carbohydrate (WSC) concentrations in leaf and stem of tall fescue and lucerne in autumn and to determine the impact of these changes on the rate and the extent of in vitro fermentation. Vegetative herbage of lucerne and tall fescue were sampled at 0900, 1300 and 1700 h, following three consecutive regrowths between April and May. Whole plants, leaf and stem were analysed for chemical composition and in vitro gas production. There was a linear increase in WSC throughout the day for all plants and plant components (P < 0.001). In both fescue and lucerne, this rise resulted in an increased rate of gas production, although this relationship was more pronounced in fescue. Use of time of day to govern grazing management decisions to potentially improve the rumen supply of WSC and nitrogen in autumn can be achieved from tall fescue or lucerne pastures. © 2015 The Royal Society of New Zealand
650 $aFESTUCA ARUNDINACEA
650 $aMEDICAGO SATIVA
653 $aHARVESTING TIME
653 $aRUMINAL FERMENTATION
653 $aSUGARS
700 1 $aBRITOS, A.
700 1 $aERRANDONEA, N.
700 1 $aGUTIÉRREZ, L.
700 1 $aCOZZOLINO, D.
700 1 $aREPETTO, J.L.
773 $tNew Zealand Journal of Agricultural Research, 2015$gv. 58, no.3, p. 281-291.
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